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mouse ultrasensitive insulin enzyme linked immunosorbent assay elisa kit  (ALPCO)


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    ALPCO mouse ultrasensitive insulin enzyme linked immunosorbent assay elisa kit
    Mouse Ultrasensitive Insulin Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by ALPCO, used in various techniques. Bioz Stars score: 96/100, based on 1123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ultrasensitive+mouse+insulin+elisa+kits/Mouse+Ultrasensitive+Insulin+ELISA/10__1016_slash_j__jff__2026__107474-106-7-20
    Average 96 stars, based on 1123 article reviews
    mouse ultrasensitive insulin enzyme linked immunosorbent assay elisa kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Placental mTOR signalling links mitochondrial dysfunction, nutrient transport and neonatal beta cell perturbations in mice
    Article Snippet: .. Insulin concentrations were determined using Ultrasensitive Mouse Insulin ELISA kits (ALPCO) and normalised by DNA determined using a PicoGreen dsDNA assay. ..

    Article Title: Accumulation of microbial DNAs promotes to islet inflammation and β cell abnormalities in obesity in mice.
    Article Snippet: .. Insulin concentrations in the supernatant were determined using Ultrasensitive mouse insulin ELISA kits (Alpco, Cat. No. 80-INSHU-E01.1). ..

    Article Title: Adipose tissue macrophages orchestrate β cell adaptation in obesity through secreting miRNA-containing extracellular vesicles
    Article Snippet: .. Insulin concentrations in the supernatant were determined using Ultrasensitive mouse insulin ELISA kits (Alpco, Cat. No. 80-INSHU-E01.1). ..

    Article Title: Placental mTOR signalling links mitochondrial dysfunction, nutrient transport and neonatal beta cell perturbations in mice.
    Article Snippet: .. Insulin concentrations were determined using Ultrasensitive Mouse Insulin ELISA kits (ALPCO) and normalised by DNA determined using a PicoGreen dsDNA assay. ..

    Article Title: Endoplasmic reticulum stress contributes to NMDA-induced pancreatic β-cell dysfunction in a CHOP-dependent manner.
    Article Snippet: Glucose-stimulated insulin secretion (GSIS) For GSIS assays, MIN6 cells or islets were incubated for 1 h in KRB buffer (2.6 mM CaCl22H2O, 1.2 mM MgSO4·7H2O, 1.2 mM KH2PO4, 4.9 mM KCl, 98.5 mM NaCl, and 25.9 mM NaHCO3, all from Sigma-Aldrich) supplemented with 20 mM HEPES (Sigma-Aldrich) and 0.1% BSA (Sigma-Aldrich) and then incubated for 1 h in KRB buffer with 2.8 mM or 16.7 mM glucose. .. The insulin concentration in the supernatants was determined using ultrasensitive mouse insulin ELISA kits (Alpco, USA) (Huang et al., 2017a, Huang et al., 2017c). .. CHOP siRNA transient transfection In vitro, transient transfection of CHOP siRNA (sc-35438, Santa Cruz, USA) was carried to knockdown CHOP according to a previous study (Dai et al., 2016).

    Article Title: Sex Differences in Pancreatic β-Cell Physiology and Glucose Homeostasis in C57BL/6J Mice
    Article Snippet: .. Naïve untested insulin content was measured from 10 islets from each mouse, using Ultrasensitive Mouse Insulin ELISA kits (ALPCO, AB_2792981) and normalized by DNA content with PicoGreen dsDNA assay kit. .. Perifusion experiments were performed using a PERI4-V2 machine (Biorep Technologies, Miami, FL, USA).

    Article Title: Accumulation of microbial DNAs promotes to islet inflammation and β cell abnormalities in obesity in mice
    Article Snippet: .. Insulin concentrations in the supernatant were determined using Ultrasensitive mouse insulin ELISA kits (Alpco, Cat. No. 80-INSHU-E01.1). ..

    Article Title: Sex Differences in Pancreatic β-Cell Physiology and Glucose Homeostasis in C57BL/6J Mice
    Article Snippet: .. Insulin concentrations were determined using commercially available Ultrasensitive Mouse Insulin ELISA kits (ALPCO, AB_2792981). ..

    Picogreen Assay:

    Article Title: Placental mTOR signalling links mitochondrial dysfunction, nutrient transport and neonatal beta cell perturbations in mice
    Article Snippet: .. Insulin concentrations were determined using Ultrasensitive Mouse Insulin ELISA kits (ALPCO) and normalised by DNA determined using a PicoGreen dsDNA assay. ..

    Article Title: Placental mTOR signalling links mitochondrial dysfunction, nutrient transport and neonatal beta cell perturbations in mice.
    Article Snippet: .. Insulin concentrations were determined using Ultrasensitive Mouse Insulin ELISA kits (ALPCO) and normalised by DNA determined using a PicoGreen dsDNA assay. ..

    Article Title: Sex Differences in Pancreatic β-Cell Physiology and Glucose Homeostasis in C57BL/6J Mice
    Article Snippet: .. Naïve untested insulin content was measured from 10 islets from each mouse, using Ultrasensitive Mouse Insulin ELISA kits (ALPCO, AB_2792981) and normalized by DNA content with PicoGreen dsDNA assay kit. .. Perifusion experiments were performed using a PERI4-V2 machine (Biorep Technologies, Miami, FL, USA).

    Concentration Assay:

    Article Title: Endoplasmic reticulum stress contributes to NMDA-induced pancreatic β-cell dysfunction in a CHOP-dependent manner.
    Article Snippet: Glucose-stimulated insulin secretion (GSIS) For GSIS assays, MIN6 cells or islets were incubated for 1 h in KRB buffer (2.6 mM CaCl22H2O, 1.2 mM MgSO4·7H2O, 1.2 mM KH2PO4, 4.9 mM KCl, 98.5 mM NaCl, and 25.9 mM NaHCO3, all from Sigma-Aldrich) supplemented with 20 mM HEPES (Sigma-Aldrich) and 0.1% BSA (Sigma-Aldrich) and then incubated for 1 h in KRB buffer with 2.8 mM or 16.7 mM glucose. .. The insulin concentration in the supernatants was determined using ultrasensitive mouse insulin ELISA kits (Alpco, USA) (Huang et al., 2017a, Huang et al., 2017c). .. CHOP siRNA transient transfection In vitro, transient transfection of CHOP siRNA (sc-35438, Santa Cruz, USA) was carried to knockdown CHOP according to a previous study (Dai et al., 2016).



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    Screening GLP-1 secretagogues from traditional Chinese medicine library by using a GLP-1-NanoLuciferase reporter GLUTag cell line. (A) Schematic of construction of GLP-1-NanoLuciferase reporter GLUTag cell line. (B) NanoLuciferase GLUTag cells were incubated with assay buffer containing DMSO (control), 20 mΜ Glucose, 10 μΜ forskolin and 50 mM KCl. Luciferase activity (luminescence) was immediately detected, secreted physiological GLP-1 were measured with GLP-1 <t>ELISA</t> kit (n = 5). *** p < 0.001 or ### p < 0.001 vs. the control group by one-way ANOVA with Turkey’s test. (C) Similar fold changes of secreted NanoLuciferase activity and corresponding physiological GLP-1 concentrations in response to different secretagogues. (D) Strategy flowchart for screening compounds that stimulate GLP-1 secretion by using reporter cell line. (E,F) NanoLuciferase activity assay for GLUTag cells treated with assay buffer plus DMSO (control) or 10 μM different compounds, compounds that exhibited a greater than 1.2-fold increase with statistic significant were shown, the red dotted line represented the values of the control group (E) , while cell viability was detected by CCK-8 assay (F) (n = 3). * p < 0.05 by Mann-Whitney U-test. (G-H) Chemical structure of hesperetin (G) and naringenin (H) . Data are expressed as the mean ± SEM.
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    Image Search Results


    Screening GLP-1 secretagogues from traditional Chinese medicine library by using a GLP-1-NanoLuciferase reporter GLUTag cell line. (A) Schematic of construction of GLP-1-NanoLuciferase reporter GLUTag cell line. (B) NanoLuciferase GLUTag cells were incubated with assay buffer containing DMSO (control), 20 mΜ Glucose, 10 μΜ forskolin and 50 mM KCl. Luciferase activity (luminescence) was immediately detected, secreted physiological GLP-1 were measured with GLP-1 ELISA kit (n = 5). *** p < 0.001 or ### p < 0.001 vs. the control group by one-way ANOVA with Turkey’s test. (C) Similar fold changes of secreted NanoLuciferase activity and corresponding physiological GLP-1 concentrations in response to different secretagogues. (D) Strategy flowchart for screening compounds that stimulate GLP-1 secretion by using reporter cell line. (E,F) NanoLuciferase activity assay for GLUTag cells treated with assay buffer plus DMSO (control) or 10 μM different compounds, compounds that exhibited a greater than 1.2-fold increase with statistic significant were shown, the red dotted line represented the values of the control group (E) , while cell viability was detected by CCK-8 assay (F) (n = 3). * p < 0.05 by Mann-Whitney U-test. (G-H) Chemical structure of hesperetin (G) and naringenin (H) . Data are expressed as the mean ± SEM.

    Journal: Frontiers in Pharmacology

    Article Title: Hesperetin and Naringenin promote glucagon-like peptide-1 secretion from L-cell through activation of TGR5 and alleviated type 2 diabetes

    doi: 10.3389/fphar.2026.1838267

    Figure Lengend Snippet: Screening GLP-1 secretagogues from traditional Chinese medicine library by using a GLP-1-NanoLuciferase reporter GLUTag cell line. (A) Schematic of construction of GLP-1-NanoLuciferase reporter GLUTag cell line. (B) NanoLuciferase GLUTag cells were incubated with assay buffer containing DMSO (control), 20 mΜ Glucose, 10 μΜ forskolin and 50 mM KCl. Luciferase activity (luminescence) was immediately detected, secreted physiological GLP-1 were measured with GLP-1 ELISA kit (n = 5). *** p < 0.001 or ### p < 0.001 vs. the control group by one-way ANOVA with Turkey’s test. (C) Similar fold changes of secreted NanoLuciferase activity and corresponding physiological GLP-1 concentrations in response to different secretagogues. (D) Strategy flowchart for screening compounds that stimulate GLP-1 secretion by using reporter cell line. (E,F) NanoLuciferase activity assay for GLUTag cells treated with assay buffer plus DMSO (control) or 10 μM different compounds, compounds that exhibited a greater than 1.2-fold increase with statistic significant were shown, the red dotted line represented the values of the control group (E) , while cell viability was detected by CCK-8 assay (F) (n = 3). * p < 0.05 by Mann-Whitney U-test. (G-H) Chemical structure of hesperetin (G) and naringenin (H) . Data are expressed as the mean ± SEM.

    Article Snippet: Ultrasensitive Mouse Insulin ELISA kit was purchased from Mercodia (10-1249-01, Uppsala, Sweden). cAMP ELISA Kit was purchased from Elabscience (E-EL-0056, Wuhan, China).

    Techniques: Incubation, Control, Luciferase, Activity Assay, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, MANN-WHITNEY